Sven Goran Eriksson named a 24-man squad, including four central defenders, but there was no place for Southgate who has now dropped in the pecking order behind Sol Campbell, Rio Ferdinand, Jonathan Woodgate and John Terry.
The full squad is:
James (West Ham)
Robinson (Leeds)
Kirkland (Liverpool)
Mills (Leeds)
Bridge (Chelsea)
A Cole (Arsenal)
Ferdinand (Man Utd)
Terry (Chelsea)
P Neville (Man Utd)
Campbell (Arsenal)
Woodgate (Newcastle)
Beckham (Real Madrid)
Scholes (Man Utd)
Lampard (Chelsea)
Hargreaves (Bayern Munich)
Dyer (Newcastle)
Murphy (Liverpool)
Gerrard (Liverpool)
Sinclair (Man City)
J Cole (Chelsea)
Butt (Man Utd)
Owen (Liverpool)
Heskey (Liverpool)
Beattie (Southampton)
However, Boro will have one player on England duty this week, Stuart Parnaby is included in the U-21 squad for their game against the Croatians on Tuesday at Upton Park.